The study of proteins and the development of new techniques and methods of purification have been essential in the progress and advance made in biotechnology. Fast Protein Liquid Chromatography (FPLC), also known as Fast Performance Liquid Chromatography, is a form of liquid chromatography, used to analyse, separate and purify proteins and other polymers from complex mixtures.
This technique is based in the principle of separation by absorption, where the protein of interest is adhered to the stationary phase and separated from the other components of the sample. In FPLC, the mobile phase is commonly a buffer while the stationary phase is normally a resin composed of cross-linked agarose bead.
FPLC has a wide range of chromatography modes such as chromatofocusing, hydrophobic interaction, and reverse phase, based on particles with average diameter sizes in the same range. However, Ion exchange and Gel filtration are two of the most common modes of FPLC. Moreover, FPLC is most often used in the Enzymology and biology field. It helps to determinate the presence of proteins that potentially lead to disease.
This technique is based in the principle of separation by absorption, where the protein of interest is adhered to the stationary phase and separated from the other components of the sample. In FPLC, the mobile phase is commonly a buffer while the stationary phase is normally a resin composed of cross-linked agarose bead.
FPLC has a wide range of chromatography modes such as chromatofocusing, hydrophobic interaction, and reverse phase, based on particles with average diameter sizes in the same range. However, Ion exchange and Gel filtration are two of the most common modes of FPLC. Moreover, FPLC is most often used in the Enzymology and biology field. It helps to determinate the presence of proteins that potentially lead to disease.